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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is noticed in several tumors, which leads to chromosome instability, and thus may perhaps lead to tumorigenesis. This protein was found to affiliate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a significant part in endorsing mobile-cycle development within the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have already been described. Two added splice variants ended up described but in-depth nucleotide sequence facts is just not but out there.
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Blocking peptides are peptides that bind especially to your concentrate on antibody and block antibody binding. These peptide commonly includes the epitope recognized with the antibody.
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This manual outlines further possible optimization of mobile and immunoassay parameters to make sure the very best final results are...
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This information outlines further attainable optimization of mobile and immunoassay parameters to make sure the absolute best success are...
Antibodies bound to the blocking peptide no longer bind on the epitope over the target protein. By comparing the staining in the blocked antibody versus the antibody on your own, you can see which staining is distinct.
Focus on Information Cyclin E belongs to your hugely conserved cyclin household, whose customers are characterised by a remarkable periodicity in protein abundance from the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add to your temporal coordination of every mitotic function. Cyclin E types a posh with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates for the G1-S phase boundary and is particularly degraded as cells progress as a result of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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